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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
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Search results for , issue "Vol. 9 No. 2 (2022): December 2022" : 18 Documents clear
Front Cover JBBI Vol 9, No 2, December 2022
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Appendix JBBI Vol 9, No 2, December 2022: Keyword Index and Author Index Teuku Tajuddin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Preface JBBI Vol 9, No 2, December 2022: Foreword and Acknowledgement Teuku Tajuddin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

MICROPROPAGATION OF POTATO (Solanum tuberosum L.) cv. GRANOLA IN LIQUID MEDIUM USING AERATION SYSTEM FOR G0 SEED PRODUCTION Karyanti Karyanti; Hayat Khairiyah; Tati Sukarnih; Yayan Rudiyana; Isni Nasrifah; Aida Wulansari; Shinta Magdalena Septiani; Dasumiati Dasumiati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (696.111 KB) | DOI: 10.29122/jbbi.v9i2.4713

Abstract

ABSTRACT Disease-free potato seeds of high quality can be obtained via in vitro culture. The use of liquid medium during in vitro cultures might boost the number of plantlets produced, however, the problem of hyperhydricity in plantlets was often encountered. This study aimed to investigate effects of different sucrose concentrations and application of aeration system on micropropagation of potato cv. Granola using liquid medium. Aseptic nodal explants with 3-4 nodes from established in vitro cultures were subjected to MS liquid medium with a factorial treatment of three sucrose concentrations (0, 7.5, 15 g. L-1) and two culture  (with and without aeration). The results showed that MS medium with 7.5 g L-1 sucrose was the best medium to produce the highest number of shoots and nodes. Furthermore, it was found that application of aeration system in MS liquid culture decreased plantlet hyperhydricity and increased the number of shoots, number nodes, plantlet height, as well as improved plantlet morphology and vigor. Application of the aeration system in liquid medium produced 200-230 new potato plants per bioreactor in the acclimatization stage and an average of 2773.5 G0 mini tubers.   ABSTRAK Benih kentang bebas penyakit berkualitas tinggi dapat diperoleh dari kultur in vitro.  Penggunaan media cair selama kultur in vitro dapat meningkatkan jumlah planlet yang dihasilkan, namun masalah hiperhidrisitas plantlet masih sering dijumpai. Penelitian ini bertujuan mengetahui pengaruh perbedaan konsentrasi sukrosa dan penerapan sistem aerasi terhadap perbanyakan kentang cv. Granola menggunakan media cair. Eksplan dengan 3-4 nodus dari kultur in vitro ditumbuhkan pada media cair MS dengan perlakuan faktorial, faktor pertama: tiga tingkat konsentrasi sukrosa (0, 7,5, 15 g. L-1) dan faktor kedua: dua sistem kultur (dengan dan tanpa aplikasi aerasi).  Hasil menunjukkan bahwa media MS dengan 7,5 g. L-1 sukrosa merupakan media terbaik untuk menghasilkan jumlah tunas dan nodus terbanyak. Selanjutnya ditemukan bahwa penerapan sistem aerasi pada kultur cair MS menurunkan hiperhidrisitas plantlet dan meningkatkan jumlah tunas, jumlah buku, tinggi plantlet, serta memperbaiki morfologi dan vigor plantlet. Penerapan sistem aerasi pada media cair menghasilkan 200-230 tanaman kentang baru per bioreaktor dalam tahap aklimatisasi dan rata-rata 2773,5 G0 umbi mini.
APPLICATION OF RECOMBINANT TRIACYLGLYCEROL LIPASE AND CARBOXYLESTERASE ENZYMES FROM Bacillus velezensis STRAIN S3 FOR POLYESTER SURFACE MODIFICATION
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.386 KB) | DOI: 10.29122/jbbi.v9i2.5109

Abstract

ABSTRACT Enzymatic polyester surface modification can be performed with lipase and esterase enzymes. In this study, the polyester fabric modification utilized triacylglycerol lipase (TGA) and carboxylesterase (CES) recombinant enzymes. The effect of these treatments was observed by determining the hydrophilicity level, dye absorption level, hydroxyl group measurement, and fiber surface morphology. The results revealed an elevated hydrophilicity level in polyester fabric, followed by dye absorption improvement and carboxyl group increase. The water absorption times required by the fabric based on the results of TGA, CES, comparative lipase, and negative control treatments were 3±0.05 seconds, 3.5±0.07 seconds, 5±0.05 seconds, and 80±11.54 minutes, respectively. Dye absorption test in polyester fabric based on these groups mentioned above were 52±0.5, 58±0.5, 178±0.5, and 2968±290 seconds. The total hydroxyl group measurement in polyester fabric was observed at 30.9±0.09, 30.5±0.05, 28.6±0.09, and 3 meq/100 g. The SEM observation showed that the enzymatic hydrolysis could alter the porous structure and surface of the fibers.   ABSTRAK Modifikasi permukaan poliester secara enzimatis dengan enzim lipase dan esterase mengubah poliester menjadi hidrofilik. Pada penelitian ini, modifikasi permukaan poliester menggunakan enzim rekombinan Triasilgliserol lipase (TGA) dan Karboksilesterase (CES). Parameter uji meliputii hidrofilisitas, penyerapan warna, pengukuran gugus hidroksil, dan morfologi permukaan serat. Hasil hidrolisis menunjukkan peningkatan hidrofilisitas, penyerapan warna dan gugus karboksil kain poliester. Waktu penyerapan air oleh kain hasil perlakuan enzim TGA, CES, lipase pembanding dan kontrol negatif berturut-turut adalah 3±0,05, 3,5±0,07, 5±0,05 detik dan 80±11,54 menit. Uji serapan warna kain poliester pada kelompok tersebut diatas berturut-turut adalah 52±0,5, 58±0,5, 178±0,5 dan 2968±290 detik. Pengukuran jumlah gugus hidroksil pada kain poliester perlakuan TGA, CES, enzim lipase pembanding dan kontrol negatif adalah 30,9±0,09, 30,5±0,05, 28,6±0,09 dan 3 meq/100 g. Hasil SEM menunjukkan adanya perubahan struktur pori dan permukaan serat.
TOTAL LIGNIN CONTENT AND AGROMORPHOLOGICAL CHARACTER DIVERSITIES OF 30 INDONESIAN RICE (Oryza sativa L.) ACCESSIONS
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.202 KB) | DOI: 10.29122/jbbi.v9i2.5178

Abstract

ABSTRACT Lignin is one of lignocellulosic components in vascular plants, essential in plant mechanical properties, water transport, and defense against pathogens. Furthemore, lignin has been applied in various industry. This study aimed to explore variation of lignin content and its-related morphological traits of Indonesian rice accessions, providing beneficial information for breeding approaches to improve utilization characteristics of grass biomass. Therefore, the total lignin contents of thirty Indonesian rice accessions using Thioglycolic Acid Lignin (TGAL) method and their correlation to seven agromorphological characters using Pearson correlation analysis were investigated. Variation of lignin content ranged from 8.38 to 20.75% (of cell wal residue, CWR), and the average value was 13.55%. Correlation analysis showed that lignin total positively correlated with plant height, stem length, panicle length, stem diameter, total fresh weight, and panicle weight per tiller. On the other hand, the number of tillers had a significantly negative correlation to lignin contents.   ABSTRAK Lignin merupakan komponen lignoselulosa yang berperan penting pada sifat mekanis tanaman, transportasi air dan pertahanan terhadap patogen pada tanaman berpembuluh. Selain itu, lignin telah banyak diaplikasikan di berbagai jenis industri. Tujuan penelitian ini untuk mengeksplorasi variasi kandungan lignin dan sifat-sifat agromorfologi aksesi padi Indonesia yang terkait, serta memberikan informasi yang bermanfaat untuk pendekatan pemuliaan sebagai pengembangan karakteristik pemanfaatan biomassa rumput-rumputan. Oleh karena itu, dilakukan pencarian informasi kandungan lignin pada tiga puluh aksesi padi Indonesia menggunakan metode Thioglycolic Acid Lignin (TGAL) dan korelasinya terhadap tujuh karakter agromorfologi menggunakan analisis korelasi Pearson. Keragaman kandungan lignin yang diperoleh adalah berkisar 8,38–20,75% (Cell Wall Residue, CWR), dengan nilai rata-rata 13,55%. Analisis korelasi menunjukkan bahwa akumulasi lignin pada batang padi berkorelasi positif dengan karakter tinggi tanaman, panjang batang, panjang malai, diameter batang, bobot total segar dan bobot malai per anakan. Sebaliknya, karakter jumlah anakan berkorelasi negatif signifikan terhadap kandungan lignin.
OPTIMIZATION OF Bacillus paramycoides FERMENTATION MEDIUM TO INCREASE THE PRODUCTION OF 5-AMINOLEVULINIC ACID IN A 10 LITER FERMENTER Dicky Adihayyu Monconegoro; Dea Indriani Astuti; Neil Priharto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (705.166 KB) | DOI: 10.29122/jbbi.v9i2.5351

Abstract

ABSTRACT 5-Aminolevulinic acid is an essential precursor for the biosynthesis of tetrapyrrole compounds, such as chlorophyll and heme. 5-ALA has the potential to be used as a plant growth and antioxidant activity enhancer. 5-ALA can be produced through fermentation by Bacillus paramycoides. This study aimed to optimize B. paramycoides fermentation medium to increase 5-ALA production. The optimization was carried out using response surface method (RSM) experimental design. 5-ALA production in a 10 L fermenter was conducted using an optimized medium and supplemented with MSG as a precursor and wood vinegar as an inhibitor. The results showed that the best medium composition was 27.78 g L-1 molasses; 9.145 g L-1 urea; 8.838 g L-1 NaCl; and 32.07 g L-1 glucose, resulting in 10.749 (log CFU mL-1) and 255.30 µM 5-ALA. 5-ALA production trial in a 10 L fermenter produced 581.82 µM 5-ALA. Medium optimization and precursor-inhibitors addition in the fermentation increased the 5-ALA yield 3.2 times compared to before optimization.   ABSTRAK 5-Aminolevulinic acid (5-ALA) merupakan prekursor penting dalam pembentukan senyawa tetrapirol seperti klorofil dan heme. 5-ALA memiliki potensi sebagai senyawa pemacu pertumbuhan dan peningkatan kandungan antioksidan tanaman. 5-ALA dapat dihasilkan melalui fermentasi oleh Bacillus paramycoides. Tujuan dari penelitian ini adalah melakukan optimasi media fermentasi B. paramycoides untuk meningkatkan konsentrasi 5-ALA. Optimasi media fermentasi dilakukan dengan menggunakan desain eksperimen response surface method (RSM). Setelah itu, dilakukan uji coba produksi 5-ALA pada fermentor 10 L menggunakan media hasil optimasi RSM dan suplementasi prekursor MSG dan inhibitor asap cair. Hasil optimasi media RSM menunjukkan bahwa komposisi media terbaik adalah 27,78 g L-1 molase; 9,145 g L-1 urea; 8,838 g L-1 NaCl; dan 32,07 g L-1 glukosa dengan jumlah sel (log CFU mL-1) sebesar 10,749 dan konsentrasi 5-ALA sebesar 255,30 µM. Uji coba produksi 5-ALA pada fermentor 10 L menghasilkan konsentrasi 5-ALA sebesar 581,82 µM. Optimasi media dan penambahan prekursor-inhibitor pada fermentasi dapat meningkatkan produksi 5-ALA sebanyak 3,2 kali lipat dibandingkan sebelum optimasi.
Back Cover JBBI Vol 9, No 2, December 2022 .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

ANTIOXIDANT EFFECT OF MORINGA (Moringa oleifera) LEAF ETHANOL EXTRACT ON SPERMATOGENESIS IN OLD WISTAR RATS (Rattus norvegicus) Luh Putu Widiastini; I Gusti Agung Manik Karuniadi; Made Tangkas
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.833 KB) | DOI: 10.29122/jbbi.v9i2.5418

Abstract

ABSTRACT Infertility is the failure of pregnancy after regular sexual intercourse for 6-12 months without contraception. Infertility in man is primarily caused by damaged sperm production, for example, impairments in the spermatogenesis process, low spermatozoa concentrations, morphological factors, and abnormal sperm motility. The purpose of this study is to ascertain the antioxidant effect of moringa (Moringa oleifera) leaves ethanol extract on spermatogenesis (spermatogonia, spermatocytes, and spermatids counts) in old Wistar rats (Rattus norvegicus). This study took healthy old Wistar rats aged 18-19 months with a body weight of 200-250 g and with no physical disabilities. A total of 36 was Wistar rats divided into two groups, namely the treatment group (supplied with Moringa leaf ethanol extract of 50 mg/kgBW/ and 0.5% Carboxymethyl cellulose (CMC) of 0.5 mL per day) and the control group (only with 0.5% CMC of 0.5 mL per day) for 30 days. The results showed that there was a significant difference in the spermatogonia, spermatocyte, and spermatid counts between the group treated with Moringa leaf ethanol extract and the control group, resulting in a p-value of 0.000. Therefore, it can be concluded that Moringa leaf ethanol extract had a significant influence on the spermatogonia, spermatocyte, and spermatid counts in old Wistar rats.   ABSTRAK Infertilitas adalah kegagalan kehamilan setelah melakukan hubungan seksual secara teratur selama 6-12 bulan tanpa alat kontrasepsi. Penyebab infertilitas pada pria disebabkan karena produksi sperma yang rusak misalnya, gangguan pada proses spermatogenesis, konsentrasi spermatozoa rendah, morfologi serta motilitas sperma yang abnormal. Penelitian ini bertujuan untuk mengetahui efek antioksidan ekstrak etanol daun kelor (Moringa oleifera) terhadap spermatogenesis (Spermatogonia, Spermatosit, dan Spermatid) pada tikus (Rattus norvegicus) galur Wistar tua. Tikus yang digunakan adalah tikus tua yang berusia 18-19 bulan dengan berat badan 200-250 g, dengan kondisi sehat dan tidak cacat fisik sejumlah 36 dibagi menjadi 2 kelompok, yaitu kelompok perlakuan (ekstrak etanol daun kelor 50 mg/kgBW/0,5 mL Carboxymethyl cellulose (CMC) 0.5% per hari) dan kelompok kontrol (CMC 0.5% 0.5 mL per hari) selama 30 hari. Hasil penelitian menunjukkan terdapat perbedaan jumlah spermatogonia, spermatosit, dan spermatid yang signifikan antara kelompok yang diberikan ekstrak etanol daun kelor dengan kelompok kontrol yang tidak diberikan dengan nilai p 0,000, sehingga dapat simpulkan ekstrak etanol daun kelor dapat memberikan pengaruh signifikan terhadap jumlah spermatogonia, spermatosit, dan spermatid pada tikus galur Wistar tua.
UTILIZATION OF SAGO DREGS AS RUMINANT FEED BY USING THE FERMENTATION METHOD: LITERATURE REVIEW Titi Lahanda Susanti; Ratu Safitri; Abun Hasbuna Padmadijaya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (565.688 KB) | DOI: 10.29122/jbbi.v9i2.5464

Abstract

ABSTRACT Every year there is a change in the stock of ruminant feed that occurs in the rainy season and water shortages in the dry season. Utilization of agricultural waste as an alternative feed is one way to overcome these problems. One of the wastes that have the potential to be used as feed ingredients is sago waste. Sago pulp is a waste that is rich in lignocellulose, namely cellulose. Several biotechnology applications in ruminant feed fermentation can improve properties such as taste, aroma, shelf life, texture and nutritional value of food. Fermentation using mold or yeast, as well as bacteria or a mixture of various microorganisms can increase the nutrients in the feed needed by ruminant feed. Processing of lignocellulosic materials is required to obtain optimal degradation results. The degradation process will convert lignocellulosic material into raw materials that are easily digested by the ruminant. Enzymes produced by microorganisms can increase crude protein, crude fat, carbohydrates, crude fiber, vitamins and minerals. Thus, the fermentation method of sago dregs and its use as feed can increase the nutritional value, so that productivity can be increased when given as feed.   ABSTRAK Setiap tahun terjadi perubahan stok pakan ternak ruminansia yaitu pada musim hujan dan kekurangan air pada musim kemarau. Pemanfaatan limbah pertanian sebagai pakan alternatif merupakan salah satu cara untuk mengatasi permasalahan tersebut. Salah satu limbah yang berpotensi untuk dimanfaatkan sebagai bahan pakan adalah limbah sagu. Ampas sagu merupakan limbah yang kaya akan lignoselulosa yaitu selulosa. Beberapa aplikasi bioteknologi dalam fermentasi pakan ternak ruminansia dapat meningkatkan sifat-sifat seperti rasa, aroma, umur simpan, tekstur dan nilai gizi makanan. Fermentasi menggunakan kapang atau khamir, serta bakteri atau campuran berbagai mikroorganisme dapat meningkatkan nutrisi dalam pakan yang dibutuhkan pakan ruminansia. Pengolahan bahan lignoselulosa diperlukan untuk mendapatkan hasil degradasi yang optimal. Proses degradasi akan mengubah bahan lignoselulosa menjadi bahan baku yang mudah dicerna oleh ruminansia. Enzim yang dihasilkan oleh mikroorganisme dapat meningkatkan protein kasar, lemak kasar, karbohidrat, serat kasar, vitamin dan mineral. Dengan demikian, metode fermentasi ampas sagu dan pemanfaatannya sebagai pakan dapat meningkatkan nilai gizi, sehingga produktivitas dapat meningkat bila diberikan sebagai pakan.

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